Journal: Frontiers in Cell and Developmental Biology
Article Title: LuHui Derivative, A Novel Compound That Inhibits the Fat Mass and Obesity-Associated (FTO), Alleviates the Inflammatory Response and Injury in Hyperlipidemia-Induced Cardiomyopathy
doi: 10.3389/fcell.2021.731365
Figure Lengend Snippet: LHD protects against hyperlipidemia-induced cardiac dysfunction. (A) Chemical structure of LHD. (B) Wistar rat feeding schedule. (C) Measurement of TC, TG, LDL-C, and HDL-C in the serum of rats fed a high-fat diet (HFD) for 10 weeks. *** p < 0.001, compared with the CTL group; # p < 0.05, ## p < 0.01, ### p < 0.001, compared with the HFD group, n = 8. (D) Oil Red O staining to identify lipid deposition in cardiac tissue (10× magnification). n = 4. (E,F) Echocardiographic analysis of left ventricular ejection fraction (EF%) and left ventricular shortening fraction (FS%) in rats fed with HFD for 10 weeks. ** p < 0.01, compared with the CTL group; # p < 0.05, compared with the HFD group, n = 8. (G) ELISA analysis of IL-6 and TNF-α in the serum. *** p < 0.001, compared with the CTL group; ### p < 0.001, compared with the HFD group. n = 8. (H) H&E staining to identify infiltrative inflammation in cardiac tissue (10× magnification), n = 4.
Article Snippet: The high-fat diet (HFD) feed, consisting of 77.6% maintenance feed, 10% lard, 10% yolk powder, 2% cholesterol, 0.2% bile salt, and methylthiouracil 0.2%, were purchased from HuaFuKang Biotechnology (Beijing, China).
Techniques: Staining, Enzyme-linked Immunosorbent Assay